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УДК 628.16:504.064

Sokolov D. M., Netchayev D. N.

Petrifilms (3M™ Petrifilm™) – innovative test-systems for microbial water quality monitoring


The specific features and benefits of the express method of microbiologic water quality monitoring with 3М™ Petrifilm™ are considered. 3М™ Petrifilm™ is an advanced medium standard used in Russia and abroad for microbiologic monitoring of food products, beverages and water quality. The 3М™ Petrifilm™ shelf life as long as dehydrated culture media; at the same time 3М™ Petrifilm™ is much easier to use. One of the main advantages of 3М™ Petrifilm™ is that there is no stage of culture media preparation. The main types of The 3М™ Petrifilm™ Aqua Plates for water testing are tests for determination of the number of heterotrophic bacteria (total microbial count), coliforms and enterobacteria (glucose-positive coliform bacteria), yeast and mold. The target microorganisms are determined from the growth on the selective medium, occurrence of acidification zone and gas formation. 3М™ Petrifilm™ Aqua Plates ensure valid and reliable results of analyzing drinking water by direct inoculation and membrane filtration.

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  1. MUK 4.2.2884-11. Metody mikrobiologicheskogo kontrolia ob"ektov okruzhaiushchei sredy i pishchevykh produktov s ispol'zovaniem petrifil'mov. – M.: Federal'nyi tsentr gigieny i epidemiologii Rospotrebnadzora, 2011.
  2. 3M Food Safety 3M™ Petriilm™ Aqua Plates for Water Testing. – 3M, USA, 2011.
  3. ISO 6222:1999. Water Quality – Enumeration of culturable micro-organisms – Colony count by inoculation in a nutrient agar culture medium.
  4. ISO 9308-1. Water quality – Detection and enumeration of Escherichia coli and coliform bacteria. Part 1: Membrane filtration method: 2000-09-15.
  5. Q Laboratories, Inc., Cincinnati, OH, USA study comparing 3M Petrifilm Aqua Plate performance vs. reference methods: Study presented at 2011 International Association for Food Protection (IAFP).
  6. Standard Methods for the Examination of Water and Wastewater, 20th Ed, 1998, method 9222B2a – Standard total coliform membrane filter procedure.
  7. Standard Methods for the Examination of Water and Wastewater, 20th Ed, 1998, method 9215A6a – Heterotrophic plate count.

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