bbk 000000

UDC 628.35:661.63

KOZLOV M. N., Kevbrina M. V., Nikolaev Yu. A., Dorofeev A. G., GRACHEV V. A., KAZAKOVA E. A., Aseyeva V. G., Zharkov A. V.

Single-reactor technology of nitrogen removal from wastewater


The experts of R&D Centre of «Mosvodokanal» OJSC developed high-effective technology of removing nitrogen from wastewater. The technology was tested at the Kur’ianovo wastewater treatment plant of the city of Moscow during processing filtrate of the digested sludge belt thickeners. The method is based on the use of specific Anammox-bacteria that oxidize ammonium with nitrite under anoxic conditions. Filtrate is processed in a sequencing batch reactor with complete mixing (SBR-reactor). Partial nitrification processes (ammonium oxidation into nitrite), ammonium oxidation with nitrite and organic matter oxidation are carried out in one reactor. Retention of extremely slow growing biomass of Anammox-bacteria has been a critical problem for all the reactors. In this particular case the problem was solved with bacteria adhesion on the reactor walls and the feed as well as with flotation. To evaluate the treatment efficiency standard methods were used. The developed single-reactor modification of the anoxic oxidation of ammonium differs from the well-known by the following parameters: high nitrogen removal efficiency, the method of biomass bacteria retention in the reactor, the availability of new Anammox bacteria, heterotrophic denitrification. All other conditions being equal the integration of Anammox single-reactor technology requires the size of the facilities ten times smaller than double-reactor technology; for this reason it is more preferable for implementation. The new technology was given the name AUTOHETAMMOX.

Key words

, , , ,

The further text is accessible on a paid subscription.
For authorisation enter the login/password.
Or subscribe


  1. Kuba T., Van Loosdrecht M. C. M., Heijnen J. J. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system. Water Research, 1996, v. 30, no. 7, pp. 1702–1710.
  2. Jetten M. S. M., Strous M., Van de Pas-Schoonen K. T., Schalk J., Van Dongen U. G. J. M., Van de Graaf A. A., Logemann S., Muyzer G., Van Loosdrecht M. C. M., Kuenen J. G. The anaerobic oxidation of ammonium. FEMS Microbiology Reviews, 1999, v. 22, pp. 421–437.
  3. Van Hulle S. W. H., Vandeweyer H. J. P., Meesschaert B. D., Vanrolleghem P. A., Dejans P., Dumoulin A. Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams. Chemical Engineering Journal, 2010, v. 162, pp. 1–20.
  4. Schmid M., Walsh K., Webb R., Rijpstra W. I. C., Van de Pas-Schoonen K., Verbruggen M. J., Hill T., Moffett B., Fuerst J., Schouten S., Sinninghe Damst J. S., Harris J., Shaw P., Jetten M., Strous M. Candidatus «Scalindua brodae», sp. nov., Candidatus «Scalindua wagneri», sp. nov., Two new species of anaerobic ammonium oxidizing bacteria. Systematic and Applied Microbiology, 2003, v. 26, pp. 529–538.
  5. Khramenkov S. V., Kozlov M. N., Kevbrina M. V., Dorofeev A. G., Kazakova E. A., Grachev V. A., Kuznetsov B. B., Poliakov D. Iu., Nikolaev Iu. A. [A new bacteria providing for anaerobic oxidation of ammonium in a biological reactor processing wastewater digested sludge filtrate]. Mikrobiologiia, 2013, v. 82, no. 5, pp. 625–634. (In Russian).
  6. http://www.mi-wea.org (accessed March 28, 2014).
  7. Szatkowska B., Cema G., Plaza E, Trela J., Hultman B. A one-stage system with partial nitritation and anammox processes in the moving-bed biofilm reactor. Water Science and Technology, 2007, v. 55, no. 8–9, pp. 19–26.
  8. Dutch start-up first Anammox reactor. World Water and Environmental Engineering, 2006, March–April, 30 p.
  9. Wyffels S., Boeckx P., Pynaert K., Zhang D., Van Cleemput O., Chen G., Verstraete W. Nitrogen removal from sludge reject water by a two-stage oxygen-limited autotrophic nitrification denitrification process. Water Science and Technology, 2004, v. 49, no. 5–6, pp. 57–64.
  10. Wett B. Solved up-scaling problems for implementing deammonification of rejection water. Water Science and Technology, 2006, v. 53, no. 12, pp. 121–128.
  11. EPA 832-R-12-011. Emerging technologies for wastewater treatment and in-plant wet weather management. March 2013.
  12. Improved control and application of nitrogen cycle bacteria for ammonia removal. Water technologies: results and opportunities: Research Directorate General. Belgium, 2004, pp. 8–12.
  13. Kalyuzhnyi S., Gladchenko M., Mulder A., Versprille B. DEAMOX – new biological nitrogen removal process based on anaerobic ammonia oxidation coupled to sulphide driven conversion of nitrate into nitrite. Water Research, 2006, v. 40, no. 19, pp. 3637–3645.
  14. Danilovich D. A., Kozlov M. N., Moizhes O. V., Nikolaev Iu. A., Kazakova E. A. [Anaerobic oxidation of ammonium for removal of nitrogen from high-concentrated wastewater]. Vodosnabzhenie i Sanitarnaia Tekhnika, 2010, no. 4, pp. 49–54. (In Russian).
  15. Vlaeminck S. E., De Clippeleir H., Verstraete W. Microbial resource management of one-stage partial nitritation/Anammox. Microbial Biotechnology, 2012, v. 5, no. 3, pp. 433–448.
  16. Metodika tekhnologicheskogo kontrolia raboty ochistnykh sooruzhenii gorodskoi kanalizatsii [The technique of process monitoring of municipal wastewater treatment facilities. Moscow, Stroiizdat Publ., 1977, 304 p.].
  17. Khramenkov S. V., Pakhomov A. N., Kozlov M. N., Nikolaev Iu. A., Dorofeev A. G., Kazakova E. A. [Pat. 2492148, RF. IPC C 02 F 3/34, C 02 F 3/30. Method of oxidation of ammonium and problematic organics in wastewater under aerobic-anoxic conditions (options)]. Izobreteniia. Poleznye Modeli, 2013, no. 25.
  18. http://www.paques.nl/anammox (accessed March 28, 2014).

vstmag engfree 200x100 2

myproject msk ru

ЭТ 2024 200х200px V2

souz ingenerov 02

raww ru zng 2023 200х300

Water Week 200x200 gif ru foreign 2