Tag:phosphate accumulating bacteria



UDC 579.695+579.8

Gogina Е. S., Bazhenov V. I., Sokolova Kseniia

Bacterial composition of activated sludge: identification and visualization


The objective of this work is to describe the capabilities of a method for visualizing activated sludge bacteria based on the information from an Internet resource for developing textbooks and/or manuals. Advanced methods of microscopy of the bacterial community are considered: optical light, scanning electron (SEM), transmission electron (TEM), confocal (CLSM), fluorescence hybridization in situ (FISH), new generation sequencing (NGS). On the basis of the methods presented, examples of bacterial flocculation of activated sludge with extracellular and reserve biopolymers are given. According to the highest citation frequency in professional reference books and literature, a sample of the most typical bacteria from the composition of floccules of activated sludge was compiled. Examples of visualization of four bacteria by SEM, TEM, KLSM, FISH methods are given: Bacillus subtilis (gram-positive, spore-forming, optionally aerobic), Nitrospira defluvii (gram-negative, aerobic, chemolithotrophic, nitrite-oxidizing), Candidatus «Accumulibacter phosphatis» (gram-negative, phosphate-accumulating), Candidatus «Microthrix parvicella» (gram-positive, filamentous, actinomycete). To date, the Internet resource contains a sufficient amount of reliable data on the visualization of activated sludge bacteria, their functionality and properties related to the key processes of biological treatment, classification or taxonomy, as well as on the methods of their microscopic study. The implementation of the capabilities for identifying the bacterial composition of activated sludge and the quantitative ratio of heterotrophic, autotrophic and phosphate-accumulating biomasses provides for setting and/or calibrating models of ASM series (for example, in GPS-X, BioWin, etc. software environment).

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UDC 628.356:661.63

KOZLOV M. N., KAZAKOVA E. A., Kharkina O. V., Dorofeev A. G., Nikolaev A. V.

A New Factor of Control of Facilities for Biological Removal of Phosphates from Wastewater


A new technological indicator making it possible to improve the efficiency and stability of phosphates removal at the facilities of biological treatment of wastewater for biogenic elements – the periodicity of change of phosphates concentration in the end of the anaerobic technological zone – is described. The use of this indicator reflecting the activity of phosphate accumulating bacteria has been assumed as a basis of a new algorithm of control of treatment facilities using the technology of biogenic elements removal and led to improvement of the efficiency of phosphates removal by 1,5–1,7 times.

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vstmag engfree 200x100 2

Баннер конференции для ВСТ 3

ecwatech2023 vst200

Wasma23 200x100 stand

myproject msk ru

Российская ассоциация водоснабжения и водоотведения

souz ingenerov 02